Journal:

Article Title: Dominant Activation of the Hedgehog Signaling Pathway in the Ovary Alters Theca Development and Prevents Ovulation

doi: 10.1210/me.2008-0391

Figure Lengend Snippet: Results of microarray analysis of ovarian RNA from Amhr2 cre /+ SmoM2 (mutant) and Amhr2 +/+ SmoM2 (control) mice, and confirmation by quantitative RT-PCR

Article Snippet: The between-plate coefficients of variation ranged from 1% to 16%; the mean was 10.6 ± 1.4%. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay identification a Exons b Smo Smoothened homolog Mm01162710_m1 8–9 Ihh Indian hedgehog Mm00439613_m1 2–3 Ptch1 Patched homolog 1 Mm00436026_m1 17–18 Gli1 GLI-Kruppel family member GLI1 Mm00494645_m1 2–3 Gli3 GLI-Kruppel family member GLI3 Mm00492333_m1 1–2 Hhip Hedgehog-interacting protein Mm00469580_m1 12–13 Spp Secreted phosphoprotein 1 Mm00436767_m1 2–3 Ptgs2 Prostaglandin-endoperoxidase synthase 2 Mm00478374_m1 5–6 Pgr Progesterone receptor Mm00435625_m1 4–5 Star Steroidogenic acute regulatory protein Mm00441558_m1 6–7 Lhcgr LH/CG receptor Mm00442931_m1 6–7 Actg2 Actin, γ 2, smooth muscle, enteric Mm00656102_m1 7–8 Cnn1 Calponin 1 Mm00487032_m1 1–2 Des Desmin Mm00802455_m1 1–2 Tagln Transgelin Mm00441660_m1 1–2 Tnc Tenascin C Mm00495662_m1 5–6 Edn2 Endothelin 2 Mm00432983_m1 3–4 18S rRNA 4319413E Open in a separate window a Taqman Gene Expression Assays (Applied Biosystems). b Exons in which forward and reverse primers anneal.

Techniques: Microarray, Mutagenesis, Control, Quantitative RT-PCR, Sequencing

Journal:

Article Title: Dominant Activation of the Hedgehog Signaling Pathway in the Ovary Alters Theca Development and Prevents Ovulation

doi: 10.1210/me.2008-0391

Figure Lengend Snippet: Expression of genes associated with smooth muscle (Cnn1, Des, Actg2, Tagln) or extracellular matrix (Tnc) and expression of Edn2 in granulosa cells and residual ovarian tissue from Amhr2+/+SmoM2 and Amhr2cre/+SmoM2 mice. Tissues were obtained from untreated mice (UT) or from eCG-primed mice before (0 h) or after injection of hCG. Total RNA was assayed by quantitative real-time RT-PCR. Data are mean ± sem; n = 3 granulosa cell and residual tissue preparations. Bars without common superscripts are significantly different (P < 0.05).

Article Snippet: The between-plate coefficients of variation ranged from 1% to 16%; the mean was 10.6 ± 1.4%. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay identification a Exons b Smo Smoothened homolog Mm01162710_m1 8–9 Ihh Indian hedgehog Mm00439613_m1 2–3 Ptch1 Patched homolog 1 Mm00436026_m1 17–18 Gli1 GLI-Kruppel family member GLI1 Mm00494645_m1 2–3 Gli3 GLI-Kruppel family member GLI3 Mm00492333_m1 1–2 Hhip Hedgehog-interacting protein Mm00469580_m1 12–13 Spp Secreted phosphoprotein 1 Mm00436767_m1 2–3 Ptgs2 Prostaglandin-endoperoxidase synthase 2 Mm00478374_m1 5–6 Pgr Progesterone receptor Mm00435625_m1 4–5 Star Steroidogenic acute regulatory protein Mm00441558_m1 6–7 Lhcgr LH/CG receptor Mm00442931_m1 6–7 Actg2 Actin, γ 2, smooth muscle, enteric Mm00656102_m1 7–8 Cnn1 Calponin 1 Mm00487032_m1 1–2 Des Desmin Mm00802455_m1 1–2 Tagln Transgelin Mm00441660_m1 1–2 Tnc Tenascin C Mm00495662_m1 5–6 Edn2 Endothelin 2 Mm00432983_m1 3–4 18S rRNA 4319413E Open in a separate window a Taqman Gene Expression Assays (Applied Biosystems). b Exons in which forward and reverse primers anneal.

Techniques: Expressing, Injection, Quantitative RT-PCR

Journal:

Article Title: Dominant Activation of the Hedgehog Signaling Pathway in the Ovary Alters Theca Development and Prevents Ovulation

doi: 10.1210/me.2008-0391

Figure Lengend Snippet: Quantitative real-time RT-PCR assays

Article Snippet: The between-plate coefficients of variation ranged from 1% to 16%; the mean was 10.6 ± 1.4%. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay identification a Exons b Smo Smoothened homolog Mm01162710_m1 8–9 Ihh Indian hedgehog Mm00439613_m1 2–3 Ptch1 Patched homolog 1 Mm00436026_m1 17–18 Gli1 GLI-Kruppel family member GLI1 Mm00494645_m1 2–3 Gli3 GLI-Kruppel family member GLI3 Mm00492333_m1 1–2 Hhip Hedgehog-interacting protein Mm00469580_m1 12–13 Spp Secreted phosphoprotein 1 Mm00436767_m1 2–3 Ptgs2 Prostaglandin-endoperoxidase synthase 2 Mm00478374_m1 5–6 Pgr Progesterone receptor Mm00435625_m1 4–5 Star Steroidogenic acute regulatory protein Mm00441558_m1 6–7 Lhcgr LH/CG receptor Mm00442931_m1 6–7 Actg2 Actin, γ 2, smooth muscle, enteric Mm00656102_m1 7–8 Cnn1 Calponin 1 Mm00487032_m1 1–2 Des Desmin Mm00802455_m1 1–2 Tagln Transgelin Mm00441660_m1 1–2 Tnc Tenascin C Mm00495662_m1 5–6 Edn2 Endothelin 2 Mm00432983_m1 3–4 18S rRNA 4319413E Open in a separate window a Taqman Gene Expression Assays (Applied Biosystems). b Exons in which forward and reverse primers anneal.

Techniques: Quantitative RT-PCR